Fast and accurate identification of dermatophytes by matrix-assisted laser desorption ionization-time of flight mass spectrometry: validation in the clinical laboratory.
De Bel, A l'Ollivier, C Ranque, S Detandt, M Hendrickx, Marijke
De Bel, A
l'Ollivier, C
Ranque, S
Detandt, M
Hendrickx, Marijke
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Abstract
The performance of a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains. Using a direct-deposit procedure after 3 incubation days yielded 40% correct identification. Both increasing incubation time and using an extraction procedure resulted in 100% correct identification.
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2014-09-01
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Peer reviewed scientific article
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Keywords
Arthrodermataceae, Clinical Laboratory Techniques, Dermatomycoses, Humans, specimen handling, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Time Factors