Complete development of Cryptosporidium parvum in MDBK cells.
Villacorta, I de Graaf, D Charlier, G Peeters, Johan
Villacorta, I
de Graaf, D
Charlier, G
Peeters, Johan
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Abstract
Sporozoites of Cryptosporidium parvum excysted in vitro from bovine oocysts were incubated with monolayers of Madin-Darby bovine kidney cells. The extent of parasite colonisation was monitored by light microscopy and immunofluorescence. Electron microscopy confirmed the complete development and replication of C. parvum within Madin-Darby bovine kidney cells.
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Date
1996-08-15
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Peer reviewed scientific article
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Keywords
Animals, Cattle, Cell Line, Cryptosporidiosis, Cryptosporidium parvum, Kidney, Microscopy, Electron, parasitology