The role of lipopolysaccharide anionic binding sites in aminoglycoside uptake in Stenotrophomonas (Xanthomonas) maltophilia
Vanhoof, R. Sonck, P. Hannecart-Pokorni, E.
Vanhoof, R.
Sonck, P.
Hannecart-Pokorni, E.
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Abstract
Aminoglycoside resistance was investigated in six clinical isolates of Stenotrophomonas (Xanthomonas) maltophilia by studying the uptake kinetics and by using a radiochemical method to detect aminoglycoside modifying enzymes. Furthermore, the lipopolysaccharides (LPS) were extracted and characterized by SDS-PAGE and chemical analysis. Dansyl-polymyxin displacement experiments confirmed the availability of anionic binding sites. Growing cells of the isolates bound dansyl-polymyxin but were not lysed
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1994-12-01
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Peer reviewed scientific article
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a, Agent, Agents, Aminoglycosides, analysi, analysis, Anti-Bacterial Agents, Antibiotic, article, Bacterial Outer Membrane Proteins, Belgium, Binding Sites, Brussels, Cell, cells, chemistry, Clinical, drug effects, Drug Resistance,Microbial, Electrophoresis,Polyacrylamide Gel, Enzymes, enzymology, im, Institute, IS, journal, Kinetics, Lipopolysaccharides, Membrane Proteins, metabolism, method, pharmacology, Polymyxins, Print, protein, Proteins, Research, resistance, Role, SB - IM, Sites, uptake, Xanthomonas