Knowledge of morphology is still required when identifying new amoeba isolates by molecular techniques
De Jonckheere, J.F. Gryseels, S. Eddyani, M.
De Jonckheere, J.F.
Gryseels, S.
Eddyani, M.
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Abstract
We have isolated several free-living amoeba strains from the environment in Ghana, which have internal transcribed spacers, including the 5.8S rDNA, sequences similar to sequences attributed to Vahlkampfiidae (Heterolobosea) in databases. However, morphological examination shows that the isolates belong to the Hartmannellidae (Amoebozoa). We provide evidence that the sequences in the databases are wrongly classified as belonging to a genus or species of the Vahlkampfiidae, but rather belong to strains of the genus Hartmannella
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2012-08-15
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Peer reviewed scientific article
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5.8S rDNA, Amoeba, article, AS, Belgium, Brussels, chemistry, classification, Cluster Analysis, cytology, Database, Databases, de, disease, Diseases, Dna, DNA,Protozoan, DNA,Ribosomal, DNA,Ribosomal Spacer, electronic, environment, Environmental Microbiology, evidence, Genes,rRNA, genetics, Ghana, im, Institute, IS, isolation & purification, journal, KNOWLEDGE, Molecular, Molecular Sequence Data, Morphology, Phylogeny, Research, Research Support, Rna, RNA,Protozoan, RNA,Ribosomal,5.8S, SB - IM, Sequence Analysis,DNA, Still, strain, Technique