Development of a pyrosequencing assay for rapid assessment of quinolone resistance in Acinetobacter baumannii isolates
Deccache, Y. Irenge, L.M. Savov, E. Ariciuc, M. Macovei, A. Trifonova, A. Gergova, I. Ambroise, J. Vanhoof, R. Gala, J.L.
Deccache, Y.
Irenge, L.M.
Savov, E.
Ariciuc, M.
Macovei, A.
Trifonova, A.
Gergova, I.
Ambroise, J.
Vanhoof, R.
Gala, J.L.
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Abstract
Rapid and reliable assessment of Acinetobacter baumannii resistance to quinolones was successfully achieved through pyrosequencing of the gyrA and parC quinolone-resistance determining regions. A strong correlation was found between quinolone resistance and mutations in gyrA codon 83 and/or in the parC gene (codons 80 or 84). Absence of QRDR mutations was associated with susceptibility to quinolones
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Date
2011-07-15
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Peer reviewed scientific article
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Keywords
a, Absence, acinetobacter, Acinetobacter baumannii, Acinetobacter Infections, article, assessment, Bacterial Proteins, Base Sequence, Belgian, Belgium, Brussels, Correlation, Development, Dna, DNA Gyrase, DNA Topoisomerase IV, drug effects, Drug Resistance,Bacterial, electronic, enzymology, EVALUATION, gene, genetics, Humans, im, IS, isolation & purification, journal, Laboratories, methods, Microbial Sensitivity Tests, microbiology, Molecular Sequence Data, Mutation, pharmacology, protein, Proteins, Quinolines, Quinolones, region, Research, Research Support, resistance, SB - IM, Sequence Analysis,DNA, study