Specific bovine antibody response against a new recombinant Cryptosporidium parvum antigen containing 4 zinc-finger motifs.
De Graaf, Dirk C. De Coninck, Hans Petry, Franz Eeckhout, Ilka B. Peeters, Johan
De Graaf, Dirk C.
De Coninck, Hans
Petry, Franz
Eeckhout, Ilka B.
Peeters, Johan
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Abstract
A Cryptosporidium parvum sporozoite and oocyst lambda gt11 cDNA library was screened with a hyperimmune rabbit serum that was developed against insoluble fragments of ultrasonicated oocysts. A clone named Cp22.4.1 encoding a protein of 231 amino acids with 4 zinc-finger domains characterized by a Cys-X2-Cys-X4-His-X4-Cys motif was isolated and characterized. There was a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD-TOPO-TA expression vector permitted to evaluate the antigenicity of the recombinant His-tagged antigen. This antigen was recognized by 2 out of 5 sera from Cryptosporidium immune calves and not by sera from parasite naive animals.
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2002-03-01
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Peer reviewed scientific article
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Keywords
Amino Acid Sequence, Animals, Antibodies, Protozoan, Antigens, Protozoan, Base Sequence, Cattle, Cryptosporidium parvum, Molecular Sequence Data, Protozoan Proteins, Rabbits, Recombinant Proteins, Zinc Fingers