Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes
Pirson, V. Mainil, J.G.
Pirson, V.
Mainil, J.G.
Citations
Altmetric:
Abstract
Attaching and effacing Escherichia coli is a new causal agent of diarrhea in calves. Its major virulence factors are the intimin protein, encoded by the eaeA gene, and the Shiga-like toxins, encoded by slt genes. Because the sequences of these genes are available, we selected specific primers to amplify each virulence gene so as to develop a new identification test based on multiplex amplification of virulence-associated genes. Of 30 tested strains, 14 were eaeA+, 15 were eaeA+ slt-I+, 1 was eaeA+ slt-I+ slt-II+, and 1 was eaeA+ slt-II+. The method proved in our hands to be fast and specific and in perfect correlation with the hybridization method
Description
Date
1995-12-09
Journal Title
Journal ISSN
Volume Title
Publisher
Chapter title
Publication type
Peer reviewed scientific article
Collections
Research Projects
Organizational Units
Journal Issue
Keywords
a, Adhesins,Bacterial, Agent, Animals, article, AS, Bacterial Outer Membrane Proteins, Bacterial Toxins, Bacterial Typing Techniques, Belgium, Carrier Proteins, Cattle, classification, Correlation, Diarrhea, e, Escherichia coli, Escherichia coli Proteins, factors, Faculty, gene, Genes, genetics, Hand, identification, im, IS, IT, journal, Laboratories, Medicine, Membrane Proteins, method, pathogenicity, polymerase chain reaction, Print, protein, Proteins, Research, Research Support, SB - IM, SELECTED, Shiga Toxin, Shiga Toxin 1, Shiga Toxin 2, specific, strain, Test, toxin, Universities, university, veterinary, Veterinary Medicine, virulence, Virulence Factors