Cross-reactivity of some commercially available deoxynivalenol (DON) and zearalenone (ZEN) immunoaffinity columns to DON- and ZEN-conjugated forms and metabolites.
Veršilovskis, A Huybrechts, Bart Tangni, Emmanuel Pussemier, L De Saeger, S. Callebaut, A.
Veršilovskis, A
Huybrechts, Bart
Tangni, Emmanuel
Pussemier, L
De Saeger, S.
Callebaut, A.
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Abstract
Seven commercially available deoxynivalenol (DON) and zearalenone (ZEN) immunoaffinity columns (IACs) were tested for cross-reactivity to conjugated forms (3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, DON-3-glucoside, DON-3-glucuronide, ZEN-glucosides, ZEN-glucuronide) and metabolites (de-epoxydeoxynivalenol, α-zearalenol, β-zearalenol) and nivalenol (NIV), using a semi-quantitative multi-mycotoxin ultra-performance liquid chromatography-tandem mass spectrometry method. The DON IACs showed cross-reactivity for nearly all DON derivatives tested. The ZEN IACs showed limited cross-reactivity to some of the ZEN derivatives. The IACs were evaluated for their potential use as sample clean-up for mycotoxins in serum.
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2011-12-01
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Peer reviewed scientific article
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Animals, Antibody Specificity, Cattle, Chromatography, Affinity, Chromatography, High Pressure Liquid, Immunoassay, serum, Tandem Mass Spectrometry, Trichothecenes, Zearalenone