The literature review conducted prior to this work highlighted various potential risks associated with developments and applications in the field of synthetic biology. These include modifying existing pathogens, re-creating extinct pathogens or designing new pathogens, including bacteria producing harmful effector proteins. The accelerated technological developments within this field, combined with the widespread availability of knowledge and technology, raise concerns about the potential for misuse or the possibility of an intended incident. Many recommendations have already been made on this subject. As authors of this report, we choose to build upon the literature review and other relevant literature by structuring the recommendations according to different stakeholders. Our objective is to reflect the diverse range of players involved in the governance of the biosecurity of synthetic biology, including scientists, scientific institutions and academia, publishers of scientific journals, funding bodies, professional organizations, the private sector, governments, and international bodies. We emphasize the vital need for consultation and coordination among these players.