High titre Hog Cholera virus production on Cytodex 3 microcarrier cultures.
Cay, Ann Brigitte De Smet, A Dubois, N Koenen, F.
Cay, Ann Brigitte
De Smet, A
Dubois, N
Koenen, F.
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Abstract
In an attempt to produce Hog Cholera virus (HCV) preparations of high titre, optimal growth and trypsinization conditions of PK-15 microcarrier cell cultures were defined. Infecting a PK-15 Cytodex 3 microcarrier culture with HCV increased the yield of virus more than 10 times compared with conventional monolayer culture in Roux flasks.
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Date
1989-07-01
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Peer reviewed scientific article
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Keywords
Animals, Cells, Cultured, Classical swine fever virus, Dextrans, Virus Cultivation