Design and evaluation of analogues of the bacterial cell-wall peptidoglycan motif L-Lys-D-Ala-D-Ala for use in a vancomycin biosensor
Hernout, O. Berthoin, K. Delattre, I. Tulkens, P.M. Carryn, S. Marchand-Brynaert, J.
Hernout, O.
Berthoin, K.
Delattre, I.
Tulkens, P.M.
Carryn, S.
Marchand-Brynaert, J.
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Abstract
Four small molecular receptors of vancomycin have been designed to make part of a novel biosensor device based on the FTIR-ATR detection: N-Boc (2a) or N-Ac (2b)-6-aminocaproyl-D-Ala-D-Ala and N-Boc (3a) or N-Ac (3b)-6-aminocaproyl-D-Ala-d-Ser. Using an original microbiological approach to assess the competition of compounds with the natural target of vancomycin in bacteria, EC(50) values of 6.3-8.0 x 10(-5)M (2a-b) and 7.1-9.3 x 10(-4)M (3a-b) were determined. Vancomycin:2b complex was characterized by MS
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2007-11-01
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Peer reviewed scientific article
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a, analysis, approach, approaches, article, bacteria, Belgium, Biosensing Techniques, Cell Wall, chemistry, Chimie, Chromatography,High Pressure Liquid, competition, de, Design, detection, Drug Design, Drug Evaluation,Preclinical, ET, EVALUATION, im, IS, journal, Molecular, MS, Peptidoglycan, Print, Receptor, receptors, Research, Research Support, SB - IM, Spectrophotometry,Ultraviolet, Spectroscopy,Fourier Transform Infrared, Target, use, values, Vancomycin