Detection of single cells of Cryptococcus neoformans in clinical samples by solid-phase cytometry
Bauters, T.G. Swinne, D. Stove, V. Nelis, H.
Bauters, T.G.
Swinne, D.
Stove, V.
Nelis, H.
Citations
Altmetric:
Abstract
A method based on solid-phase cytometry for the detection and enumeration of single cells of Cryptococcus neoformans in serum and cerebrospinal fluid is described. Both viable and nonviable cells are detected by using fluorescence viability labeling and immunofluorescence. This 30-min procedure has a detection limit of 3 to 6 cells per ml
Description
Date
2003-07-01
Journal Title
Journal ISSN
Volume Title
Publisher
Chapter title
Publication type
Peer reviewed scientific article
Collections
Research Projects
Organizational Units
Journal Issue
Keywords
analysi, analysis, article, Belgium, Cell, cells, cerebrospinal fluid, Clinical, Clinical samples, Cryptococcosis, Cryptococcus, cytology, detection, Diagnosis, EVALUATION, Flow Cytometry, Fluorescence, Fluorescent Antibody Technique, growth & development, Humans, im, IS, isolation & purification, journal, labeling, Laboratories, Meningitis,Cryptococcal, method, methods, microbiology, Print, Research, Research Support, Sample, Samples, SB - IM, serum, study, Time Factors, Universities, university, viability