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Characterisation of reversed-phase liquid chromatographic columns by chromatographic tests. Evaluation of 36 test parameters: repeatability, reproducibility and correlation

Visky, D.
Heyden, Y.V.
Ivanyi, T.
Baten, P.
de Beer, J.L.
Kovacs, Z.
Noszal, B.
Roets, E.
Massart, D.L.
Hoogmartens, J.
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Abstract

The European Pharmacopoeia (Ph. Eur.) or other official compendia give only a general description of the stationary phase in the description of a liquid chromatographic method. Therefore the selection of a column giving suitable selectivity presents difficulties. Earlier, a test procedure was proposed that allows measurement of a number of parameters which are reported to be representative for stationary phase characteristics. This paper describes how the test procedure was applied on 69 RP-LC C18 columns. Chromatographic parameters obtained as test results were evaluated, and their repeatability, reproducibility and correlation were examined

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2002-11-15
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Peer reviewed scientific article
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article, AS, Belgium, Chromatography,High Pressure Liquid, European, EVALUATION, general, im, instrumentation, IS, journal, measurement, method, Paper, parameters, ph, Print, Reproducibility, Reproducibility of Results, Research, Research Support, result, results, SB - IM, Spectrophotometry,Ultraviolet, study, Test, tests, VALIDATION, Validation study
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