Evaluation of the Premi Test Salmonella, a commercial low-density DNA microarray system intended for routine identification and typing of Salmonella enterica.
Wattiau, P Weijers, Thijs Andreoli, Peter Schliker, Christine Veken, Heidi Vander Maas, Henny M.E. Verbruggen, Anjo J. Heck, Max E.O.C. Wannet, Wim J.
Wattiau, P
Weijers, Thijs
Andreoli, Peter
Schliker, Christine
Veken, Heidi Vander
Maas, Henny M.E.
Verbruggen, Anjo J.
Heck, Max E.O.C.
Wannet, Wim J.
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Abstract
A new commercial system based on genetic profiling and aimed at identifying Salmonella enterica serovars was evaluated by comparing its performance with classical serotyping on 443 strains. Within 62 serovars represented, 60 gave unique genetic profiles while 2 were undistinguishable. Results were obtained within 8 h, were reproducible and clear-cut. The system allowed single-tube processing of the samples and required no peculiar technical skill. It showed interesting potential for routine laboratory testing.
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Date
2008-04-30
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Peer reviewed scientific article
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Keywords
Bacterial Typing Techniques, Consumer Product Safety, DNA, Bacterial, Food Contamination, Food Microbiology, Gene Expression Profiling, Genotype, Humans, Oligonucleotide Array Sequence Analysis, Reproducibility of Results, Salmonella enterica, Sensitivity and Specificity, Serotyping, Species Specificity